SNP discovery and validation for parentage assignment in hatchery progeny of the European abalone Haliotis tuberculata

Selective breeding strategies require pedigree information over generations, but many species produced in aquaculture are too small to be physically tagged at early stages. Consequently, maintaining a sufficient number of separate families is often needed but costly and logistically difficult. Alternatively, parentage assignment can be obtained using DNA markers. We developed a panel of single nucleotide polymorphism (SNP) markers for the European abalone Haliotis tuberculata using an existing transcriptomic resource. An initial set of 2,176,887 SNPs was filtered to select 500 for high throughput genotyping. Of these, 298 SNPs were amplified in at least 90% of our H. tuberculata samples, consisting of a mixed family cohort (945 offspring) generated by crossing 40 abalones, and 5 full-sib training families. Based on amplification success among parents, minimum allele frequency and checks carried out against the training families, a subset of 123 markers was used to carry out parentage assignment in our mixed family cohorts. Maximum likelihood and exclusion-based methods of parentage assignment yielded consistent results, allowing parentage to be assigned in 98.9% of the studied progeny. Optimization of markers suggests that the 60 most informative SNPs may be sufficient for 95% assignment success in these progeny. The panel was also used to estimate effective population size, and revealed a low Ne due to high variance of reproductive success between parents. Our panel could be used to estimate genetic parameters of traits in mixed family cohorts, an essential stage to initiate selective breeding in H. tuberculata. It could also be useful tool in the context of monitoring stock enhancement and population genetics studies.

Selective breeding strategies require pedigree information over generations, but many species produced in aquaculture are too small to be physically tagged at early stages. Consequently, maintaining a sufficient number of separate families is often needed but costly and logistically difficult. Alternatively, parentage assignment can be obtained using DNA markers. We developed a panel of single nucleotide polymorphism (SNP) markers for the European abalone Haliotis tuberculata using an existing transcriptomic resource. An initial set of 2,176,887 SNPs was filtered to select 500 for high throughput genotyping. Of these, 298 SNPs were amplified in at least 90% of our H. tuberculata samples, consisting of a mixed family cohort (945 offspring) generated by crossing 40 abalones, and 5 full-sib training families. Based on amplification success among parents, minimum allele frequency and checks carried out against the training families, a subset of 123 markers was used to carry out parentage assignment in our mixed family cohorts. Maximum likelihood and exclusion-based methods of parentage assignment yielded consistent results, allowing parentage to be assigned in 98.9% of the studied progeny. Optimization of markers suggests that the 60 most informative SNPs may be sufficient for 95% assignment success in these progeny. The panel was also used to estimate effective population size, and revealed a low Ne due to high variance of reproductive success between parents. Our panel could be used to estimate genetic parameters of traits in mixed family cohorts, an essential stage to initiate selective breeding in H. tuberculata. It could also be useful tool in the context of monitoring stock enhancement and population genetics studies.

Disciplines

Fisheries and aquaculture

Keywords

abalone, parentage assignment, pedigree, selective breeding, transcriptome, SNP

Location

48.913738N, 48.449509S, -3.282045E, -4.85309W

Data

FileSizeFormatProcessingAccess
Data file 1: Information about the 500 SNPs that were genotyped
372 KoCSVProcessed data
Data file 2: Genotypes of 1065 individual abalone for 298 SNPs
1 MoCSVProcessed data
Data description
1 KoTEXT
How to cite
Harney Ewan, Lachambre Sebastien, Roussel Sabine, Huchette Sylvain, Enez Florian, Morvezen Romain, Haffray Pierrick, Boudry Pierre (2018). SNP discovery and validation for parentage assignment in hatchery progeny of the European abalone Haliotis tuberculata. SEANOE. https://doi.org/10.17882/51704
In addition to properly cite this dataset, it would be appreciated that the following work(s) be cited too, when using this dataset in a publication :
Harney Ewan, Lachambre Sebastien, Roussel Sabine, Huchette Sylvain, Enez Florian, Morvezen Romain, Haffray Pierrick, Boudry Pierre (2018). Transcriptome based SNP discovery and validation for parentage assignment in hatchery progeny of the European abalone Haliotis tuberculata. Aquaculture. 491. 105-113. https://doi.org/10.1016/j.aquaculture.2018.03.006, https://archimer.ifremer.fr/doc/00429/54061/

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