An 18S V4 rDNA metabarcoding dataset of protist diversity in the Atlantic inflow to the Arctic Ocean, through the year and down to 1000 m depth.

Date 2014
Author(s) Egge ElianneORCID1, 2, Elferink StephanieORCID3, Vaulot DanielORCID4, 5, John UweORCID3, Bratbak GunnarORCID6, Larsen AudORCID7, Edvardsen BenteORCID1
Affiliation(s) 1 : University of Oslo, Department of Biosciences, PO Box 1066 Blindern, NO-0316 Oslo, NORWAY
2 : University of Duisburg-Essen, Fakultät für Biologie, Universitätsstr. 5, DE-45141 Essen, GERMANY
3 : Alfred-Wegener-Institut Helmholtz-Zentrum für Polar- und Meeresforschung, Am Handelshafen 12, DE-27570 Bremerhaven, GERMANY
4 : UMR7144, CNRS, Sorbonne Université, Station Biologique de Roscoff. Place Georges Teissier, FR-29682 Roscoff, FRANCE
5 : Asian School of the Environment, Nanyang Technological University, 50 Nanyang Avenue, SINGAPORE 639798
6 : University of Bergen, Department of Biological Sciences, PO Box 7803, NO-5020 Bergen, NORWAY
7 : NORCE Norwegian Research Centre AS, PO Box 7810, NO-5020 Bergen, NORWAY
DOI 10.17882/79823
Publisher SEANOE
Keyword(s) protists, metabarcoding, Arctic, 18s, rDNA, microbial eukaryotes, epipelagic zone, mesopelagic zone, seasonality, winter, spring bloom, post bloom, West Spitsbergen Current, Svalbard, Protist Ribosomal Reference Database, picoplankton, nanoplankton, microplankton, size fractions
Abstract

Arctic marine protist communities have been understudied due to challenging sampling conditions, in particular during winter and in deep waters. The aim of this study was to improve our knowledge on Arctic protist diversity through the year, both in the epipelagic (< 200 m depth)  and mesopelagic zones (200-1000 m depth). Sampling campaigns were performed in 2014, during five different months, to capture the various phases of the Arctic primary production: January (winter), March (pre-bloom), May (spring bloom), August (post-bloom) and November (early winter). The cruises were undertaken west and north of the Svalbard archipelago, where warmer Atlantic waters from the West Spitsbergen Current meets cold Arctic waters from the Arctic Ocean. From each cruise, station, and depth, 50 L of sea water were collected and the plankton was size-fractionated by serial filtration into four size fractions between 0.45-200 µm, representing the picoplankton, nanoplankton and microplankton. In addition vertical net hauls were taken from 50 m depth to the surface at selected stations. From the plankton samples DNA was extracted, the V4 region of the 18S rRNA-gene was amplified by PCR with universal eukaryote primers and the amplicons were sequenced by Illumina high-throughput sequencing. Sequences were clustered into Amplicon Sequence Variants (ASVs), representing protist genotypes, with the dada2 pipeline. Taxonomic classification was made against the curated Protist Ribosomal Reference database (PR2). Here we present the ASV table, metadata for the sequenced size-fractionated plankton samples, and environmental data from the corresponding water masses. Fastq-files with raw reads are available from the European Nucleotide Archive, under project accession number PRJEB40133.

Licence CC-BY
Acknowledgements The study was conducted as part of the Research Council of Norway supported project Micropolar - Processes and Players in Arctic Marine Pelagic Food Webs - Biogeochemistry, Environment and Climate Change no. 225956/E10 (prosjektbanken.forskningsradet.no/#/project/NFR/225956/). DV was supported by ANR contract PhytoPol (ANR-15-CE02-0007). We wish to thank members of the MicroPolar and Carbon Bridge projects for assisting in the sampling campaigns, and the crews at K/V Svalbard (January cruise), R/V Lance (March) and R/V Helmer Hanssen (May, August and November cruise)
Data
File Size Format Processing Access
ASV table of an 18s rDNA metabarcoding dataset of arctic marine protists. 4 MB XLS, XLSX Processed data Open access
Meta data for the sequenced DNA samples in the ASV table. 30 KB TEXTE Open access
Environmental data from the water masses corresponding to the size-fractionated plankton samples. 23 KB TEXTE Raw data Open access
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How to cite 

Egge Elianne, Elferink Stephanie, Vaulot Daniel, John Uwe, Bratbak Gunnar, Larsen Aud, Edvardsen Bente (2014). An 18S V4 rDNA metabarcoding dataset of protist diversity in the Atlantic inflow to the Arctic Ocean, through the year and down to 1000 m depth. SEANOE. https://doi.org/10.17882/79823