Partial raw data of the carbonate system after years of transgenerational exposure to ocean acidification in the European Sea Bass Dicentrarchus labrax

Date 2022-04
Temporal extent 2013-10-24 -2019-10-17
Author(s) Cohen-Rengifo MishalORCID1, Danion MorganeORCID2, Gonzalez Anne-Alicia3, Begout Marie-LaureORCID4, Cormier AlexandreORCID5, Noel CyrilORCID5, Cabon JoëlleORCID2, Vitre Thomas1, Mark Felix C.ORCID6, Mazurais DavidORCID1
Affiliation(s) 1 : IFREMER, PFOM-ARN, F-29280, Plouzané, France
2 : French Agency for Food, Environmental and Occupational Health and Safety (ANSES), Ploufragan-Plouzané Laboratory, Fish Viral Pathology Unit, Technopôle Brest-Iroise, 29280 Plouzané, France
3 : MGX, Biocampus Montpellier, CNRS, INSERM, University of Montpellier, Montpellier, France
4 : MARBEC, University of Montpellier, CNRS, IFREMER, IRD, 34250 Palavas-les-Flots, France
5 : IFREMER, SEBIMER, 29280 Plouzané, France
6 : Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research (AWI), Department of Integrative Ecophysiology, 27570 Bremerhaven, Germany
DOI 10.17882/87395
Publisher SEANOE
Keyword(s) raw data, carbonate system parameters, physico-chemical parameters, transgenerational ocean acidification, viral challenge
Abstract

This data set is linked to a study that sought to investigate the impacts of long-term ocean acidification on the olfactory rosette transcriptome of the European sea bass (Dicentrarchus labrax) and on viral resistance.  We exposed two generations of the D. labrax to end-of-century predicted pH levels (IPCC RCP8.5), with parents being exposed for 53 months (F1) and their offspring for 18 months (F2). Our design included a transcriptomic analysis of the olfactory rosette (collected from the F2) and a viral challenge (exposing F2 to betanodavirus) where we assessed survival rates.

This data set contains physico-chemical parameters of the rearing sea water including the carbonate system components as well as survival data and temperature measurements during the viral challenge.

The rearing periods extends as follows:

F1 parents: 24 October 2013 - 26 March 2018; in duplica tanks per treatment

F2 eggs phase: 27 March 2018 - 31 March 2018; in a simplica per treatment

F2 larval phase: 1 April 2018 – 1 June 2018; in triplica tanks per treatment

F2 juvenile phase: 2 April 2018 – 17 October 2019; in a duplica per treatment

However, since this study extends over 7 years, some data is not available in digital form but in paper form. Therefore, here we provide digitalized data for the following periods.

F1 parents: 8 February 2016 - 6 March 2018; monthly data showing physico-chemical and carbonate parameters of the sea water

F2 eggs phase: 27 March 2018 - 31 March 2018; daily data showing the pH and temperature of the sea water

F2 larval phase: 1 April 2018 – 1 June 2018; monthly data showing physico-chemical and carbonate parameters of the sea water and daily data showing the pH and temperature of the sea water

F2 juvenile phase:   2 April 2018 – 24 October 2018; daily data showing the pH and temperature of the sea water

F2 juvenile phase:   2 April 2018 – 11 February 2019; monthly data showing physico-chemical and carbonate parameters of the sea water

pH in NIST scale and temperature were daily measured with a WTW 3110 pH meter (Xylem Analytics Germany, Weilheim, Germany; with electrode: WTW Sentix 41) calibrated daily with pH4.0 and pH7.0 buffers (WTW, Germany). Total alkalinity was measured following the adapted protocol of Strickland and Parsons: a 50 ml sample of filtered tank seawater was mixed with 15 ml HCl (0.01 M) and pH was measured immediately. The software CO2SYS using the constants from Mehrbach et al. refitted by Dickson and Millero were used to calculate pH in NIST scale to total scale and the carbonate chemistry components.

Licence CC-BY
Acknowledgements The is study was supported by the AWI-MARUM-IFREMER AMI Partnership Programme (DEADLY TRIO project), LabexMer (ANR-10LABX-0019, OASYS project), the Ministry of Ecological and Solidarity Transition and the Foundation for Biodiversity Research (Ocean Acidification Program, PACIO project), the Deutsche Forschungsgemeinschaft, PE 1157/8–1, MA4271/3–1 (the FITNESS project) and France Génomique National infrastructure, funded as part of “Investissement d’Avenir” program managed by Agence Nationale pour la Recherche (contract ANR-10-INBS-09).
Data
File Size Format Processing Access
533 KB XLS, XLSX Quality controlled data Access on demand until 2023-04-01
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How to cite 

Cohen-Rengifo Mishal, Danion Morgane, Gonzalez Anne-Alicia, Begout Marie-Laure, Cormier Alexandre, Noel Cyril, Cabon Joëlle, Vitre Thomas, Mark Felix C., Mazurais David (2022). Partial raw data of the carbonate system after years of transgenerational exposure to ocean acidification in the European Sea Bass Dicentrarchus labrax. SEANOE. https://doi.org/10.17882/87395


In addition to properly cite this dataset, it would be appreciated that the following work(s) be cited too, when using this dataset in a publication :


Cohen-Rengifo Mishal, Danion Morgane, Gonzalez Anne-Alicia, Bégout Marie-Laure, Madec Lauriane, Cormier Alexandre, Noël Cyril, Cabon Joëlle, Vitré Thomas, Mark Felix C., Mazurais David (2021). The extensive intergenerational molecular effects of ocean acidification on the olfactory epithelium transcriptome of a marine fish are associated with a better viral resistance. Preprint, 33p. Publisher's official version : https://doi.org/10.21203/rs.3.rs-1197163/v1 , Open Access version : https://archimer.ifremer.fr/doc/00742/85438/