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Partial raw data: sea water carbonate system after years of transgenerational exposure to ocean acidification in the European Sea Bass Dicentrarchus labrax
This data set is linked to a study that sought to investigate the impacts of long-term ocean acidification on the olfactory rosette transcriptome of the European sea bass (Dicentrarchus labrax) and on viral resistance. We exposed two generations of the D. labrax to end-of-century predicted pH levels (IPCC RCP8.5), with parents being exposed for 53 months (F1) and their offspring for 18 months (F2). Our design included a transcriptomic analysis of the olfactory rosette (collected from the F2) and a viral challenge (exposing F2 to betanodavirus) where we assessed survival rates.
This data set contains physico-chemical parameters of the rearing sea water including the carbonate system components as well as survival data and temperature measurements during the viral challenge.
The rearing periods extends as follows:
F1 parents: 24 October 2013 - 26 March 2018; in duplica tanks per treatment
F2 eggs phase: 27 March 2018 - 31 March 2018; in a simplica per treatment
F2 larval phase: 1 April 2018 – 1 June 2018; in triplica tanks per treatment
F2 juvenile phase: 2 April 2018 – 17 October 2019; in a duplica per treatment
However, since this study extends over 7 years, some data is not available in digital form but in paper form. Therefore, here we provide digitalized data for the following periods.
F1 parents: 8 February 2016 - 6 March 2018; monthly data showing physico-chemical and carbonate parameters of the sea water
F2 eggs phase: 27 March 2018 - 31 March 2018; daily data showing the pH and temperature of the sea water
F2 larval phase: 1 April 2018 – 1 June 2018; monthly data showing physico-chemical and carbonate parameters of the sea water and daily data showing the pH and temperature of the sea water
F2 juvenile phase: 2 April 2018 – 24 October 2018; daily data showing the pH and temperature of the sea water
F2 juvenile phase: 2 April 2018 – 11 February 2019; monthly data showing physico-chemical and carbonate parameters of the sea water
pH in NIST scale and temperature were daily measured with a WTW 3110 pH meter (Xylem Analytics Germany, Weilheim, Germany; with electrode: WTW Sentix 41) calibrated daily with pH4.0 and pH7.0 buffers (WTW, Germany). Total alkalinity was measured following the adapted protocol of Strickland and Parsons: a 50 ml sample of filtered tank seawater was mixed with 15 ml HCl (0.01 M) and pH was measured immediately. The software CO2SYS using the constants from Mehrbach et al. refitted by Dickson and Millero were used to calculate pH in NIST scale to total scale and the carbonate chemistry components.
Disciplines
Chemical oceanography, Environment, Fisheries and aquaculture
Keywords
raw data, carbonate system parameters, physico-chemical parameters, transgenerational ocean acidification, viral challenge
Location
48.35N, 48.35S, -4.56E, -4.56W
Data
File | Size | Format | Processing | Access | |
---|---|---|---|---|---|
Data file | 876 Ko | XLS, XLSX | Quality controlled data |