Eurofleets+ CARBO-ACID cruise (SEA02_10) Datasets

The CARBO-ACID research cruise (EUROFLEETS+ SEA02_10) was carried out on the RV Ramón Margalef between August 2nd and August 11st, with departing from Vigo – Spain and ending in Lisbon – Portugal. The main objective of this cruise was to collect data and samples to study the potential effects of ocean acidification on carbonate marine organisms (coccolithophores, pteropods, planktonic and benthic foraminifera, and corals) along the Iberian margin. With this objective, oceanographic data and water samples, plankton, cold-water corals and sediment samples were collected during an upwelling season, along two transects coinciding with the two persistent upwelling filaments off the Iberia Margin: the Cape Finisterra and the Cape Roca. In this dataset is guiven all the acquired data recollected onboad. 

During the CARBO-ACID cruise we did a total of 7 stations, 4 stations along the Cape Finisterra transect (from W to E: CA3, CA2, CA7, CA8) and 3 stations at the Cape Roca (from W to E: CA6, CA5, CA4) transect (Fig). At each station we usually started with a multibeam survey, a CTD and Rosette cast. These initial operations allowed to identify the different water masses present in this area, characterize their physical properties and to recover seawater samples at specific depth levels. The seawater samples were onboard subsampled, preserved in cold conditions or with chemicals and/ or filtered for several further analysis in the shore-based laboratories: DNA, chlorophyll, fitoplankton, coccolithophores, pH, alkalinity, stable isotopic composition, trace elements concentration and Suspend Particulate Matter. Subsequently to these operations, at each station, two vertical tows with a plankton multinet (with 5 nets) were done on the top 700 m of the water column to sample the planktonic communities of the different water depths. After this, sediment samples were recovered with a box-corer to study the past oceanographic conditions, between the pre-industrial Era and the Present, with multi-proxies used in paleoceanography and sedimentology. A total of 10 box-cores were recollected and each of them was onboard sub-sampled for eDNA, enzymes and benthic foraminifera. Fifteen shipek grab samples were recollected at the Fontanelas seamount (Estremadura Spur), station CA6, to characterize the sedimentary cover and to evaluate the presence of deep cold-water corals.
Preliminary results show that the stations CA7, CA8 and CA4, located close to the coast, as expected, are the most influenced by the coastal upwelling, exhibiting colder surface water, higher values of fluorescence, and more zooplankton content reflecting higher phyto-zooplankton concentrations, as typical of the upwelling waters. At station CA4 temperature was higher and fluorescence showed lower values, indicative of less phytoplankton, and interpreted as indicating a different upwelling source water from that upwelled further north. Based on the CTD data, the Cape Roca transect is more influenced by the subtropical East North Atlantic Central Water (ENACWst), while the Cape Finisterra transect is more under the influence of the subpolar branch (ENACWsp). Seafloor sediment samples showed significant differences between the stations. Along the northern transect (Cape Finisterra) the seafloor sediments show an increase in grain size from the offshore to the coast. The offshore stations CA3 and CA2 revealed finer grained sediments, CA8 were composed of coarser sand and the station CA7, the shallowest station 77 m, presented the sediment composed mainly of shell fragments and coarse grain sand. Along the southern transect (Cape Roca), the offshore station CA6 (Fontanelas seamount) has coarser sandy sediments with rock clasts and cold-water coral fragments, and the stations CA5 and CA4 with fine sand to muddy sediments. The detailed CA6 bathymetry allowed to verify the existence of small plateaus on the slope of the Fontanelas seamount, where the fossil cold-water corals fragments were found, suggesting that this area is a very interesting system deserving further study with a ROV, and to characterize the corals fields and verify if there are live corals.
These recollected data and samples will allow not only to reconstruct the pH variability under different environmental conditions, but also to estimate the biogeochemical changes along the coastal ocean waters as the anthropogenic influence increases. These results will contribute to better understand and model the effects on the biota under the future expected oceans pH changes.


Biological oceanography, Chemical oceanography, Environment, Marine geology, Physical oceanography


Eurofleets+, Ocean acidification, Climate Change, Western Iberia margin, water masses, Upwelling


43.05N, 38.37S, -8.5E, -12.15W


Multibeam data
Instrument: Kongsberg EM710 hull-mounted multibeam echo sounder system on the RV Ramón Margalef.
Metadata and Data Processing: Multibeam data was logged in Kongsberg .all format. Data was processed onboard using the CARIS HIPS and SIPS software. The CARIS HIPS workflow is given in the CARBO-ACID cruise report. Final ASCII ArcGIS grided dataset is given in .csv format. CARBO-ACID multibeam data was also made available at the EMODnet Bathymetry. The CARBO-ACID multibeam dataset ID at the EMODnet Bathymetry is: MB_CARBOACID2022 and the bathymetry grids at 30 or 50 m resolution can be downloaded at the EMODnet Bathymetry data portal.
Variables: Latitude (DD), Longitude (DD), depth values (m), WGS84.

CTD data
Instrument: Seabird-CTD-system 2x SBE 911 Plus (s/n 09P27491-0670) installed on a 24 Niskin bottles rosette, always operated in station, vertical casts. Sensors: Conductivity - SBE 4C (s/n 90270-042688), Temperature - SBE 3plus (s/n 4169), Pressure - Max. prof. 6.885m. (s/n 0670), Fluorescence / Turbidity - WETLabs ECO-FL-NTU (s/n FLRTD-034), Dissolved oxygen - SBE 43 (s/n 90419-430270), Pomp - Max. prof. 10.500m SBE 5T (s/n 90160-053253).
Metadata and Data Processing: Raw data processed with Sea-Bird SBE Data Processing v7.26.7.1. Processing routines included: Data conversion, Data filtering, Alignment, Cell Thermal Mass, Loop, Derivative variables calculation, Bottle summary and ASCII outputs.
Variables: Are described in the heather ASCII file (.hdr). Date in Julian Day; Time [hours]; Time [minutes]; Time [seconds]; Depth [salt water, m], lat = 42.87; Pressure, Digiquartz [db]; Conductivity [S/m];  Salinity, Practical [PSU];  Specific Conductance [uS/cm];  Potential Temperature [ITS-90, deg C];  Potential Temperature Anomaly [ITS-90, deg];  Pressure Temperature [deg C]; Temperature [ITS-90, deg C]; Density [density, kg/m^3]; Density [sigma-theta, kg/m^3];  Average Sound Velocity [Chen-Millero, m/s]; Sound Velocity [Chen-Millero, m/s]; Sound Velocity [Delgrosso, m/s]; Sound Velocity [Wilson, m/s]; Thermosteric Anomaly [10^-8 * m^3/kg]; Turbidity, WET Labs ECO [NTU]; Fluorescence, WET Labs ECO-AFL/FL [mg/m^3]; Nitrogen Saturation [ml/l]; Oxygen raw, SBE 43 [V]; Oxygen, SBE 43 [ml/l]; Oxygen, SBE 43 [% saturation]; Oxygen Saturation, Garcia & Gordon [ml/l]; Oxygen Saturation, Weiss [ml/l]; Bottle Position in Carousel; Bottles Fired.

Multinet hydrographic data
Instrument and sensors: HYDRO-BIOS multi plankton sampler MultiNet, type Midi with 50 x 50 cm opening frame (0.25 m2), equipped with five nets (nets and cups with a 100 μm mesh size). Pressure sensor functional but, temperature, conductivity and flowmeter sensor (both flow in and flow out) were malfunctioning and therefore the temperature, conductivity and flow variables are not reliable.
Metadata: Data processed with OceanLab 3, the data acquisition software for HYDRO-BIOS Instruments.
Variables: Time (hh:mm:ss), Number of net, pressure (dbar), Volume (m³) only estimated values are available.

CARBO-ACID Stations List

List of all the stations and operations

Multibeam backscatter data

Instrument: Kongsberg EM710 hull-mounted multibeam echo sounder system on the RV Ramón Margalef.
Metadata and Data Processing: Multibeam data was logged in Kongsberg .all format. Backscatter data was processed on board and reprocessed onshore using the CARIS HIPS and SIPS software. Final ASCII ArcGIS grided dataset was converted and given in .xyz format. Variables: Latitude (XX) and Longitude (YY) are given in metric UTM coordinates. Z is the backscatter value. Projection: WGS84 UTM projection Zone 29N.


CARBO-ACID multibeam dataset
31 MoCSVProcessed data
CTD data
10 MoTEXTProcessed data
Multinet hydrographic data
168 KoHYDRO-BIOS OceanLab 3 dataRaw data
CARBO-ACID Stations List
4 KoCSVProcessed data
Multibeam backscatter data
10 MoTEXTRaw data
How to cite
Salgueiro Emília, Magalhães Vítor, Rebotim Andreia, Matos Lélia, Schweizer Magali, Sousa Fátima, González Martín Maria, Batista Luis Batista (2022). Eurofleets+ CARBO-ACID cruise (SEA02_10) Datasets. SEANOE.

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